Compound in Profiling

Compound Information

Test on : RBC, Millipore

Compound Name
RBC, Millipore : bisindolylmaleimide i

Inchikey
Same : QMGUOJYZJKLOLH-N
RBC, Millipore : QMGUOJYZJKLOLH-UHFFFAOYSA-N

ChemSpider
RBC, Millipore : 2303

Pubchem CID
RBC, Millipore : 2396

ChEMBL
RBC, Millipore : CHEMBL7463

	DRx	RBC	Metz	Millipore	GPCR
bioactivity	Kd (nM)	Remain Activity (%)	Ki (nM)	Remain Activity (%)	Inhibition (%)
active criteria	<3000nM	<50%	<1000nM	<50%	>50%

AGC

Test Member No. in Family
Family	no. Member on 518kinase	RBC	Millipore
PDK1	1	1	1
Akt	3	3	3
DMPK	7	5	5
GRK	7	6	3
RSK	8	8	7
PKA	5	3	2
PKC	9	9	8
PKG	2	3	-
PKN	3	2	1
SGK	3	3	3
NDR	4	1	-

AGC - PDK1

Bioactivity
Kinase	RBC	Millipore
PDK1	inactive (83.31%)	inactive (63%)

AGC - Akt

Bioactivity
Kinase	RBC	Millipore
AKT1	inactive (93.53%)	inactive (103%)
AKT2	inactive (88.72%)	inactive (107%)
AKT3	active (31.47%)	active (30%)

AGC - DMPK

Bioactivity
Kinase	RBC	Millipore
DMPK1	inactive (94.67%)	active (31%)
MRCKa	inactive (97.14%)	inactive (115%)
MRCKb	inactive (90.77%)	inactive (95%)
ROCK1	inactive (92.26%)	inactive (83%)
ROCK2	inactive (113.27%)	inactive (119%)

AGC - GRK

Bioactivity
Kinase	RBC	Millipore
BARK1	inactive (96.48%)	-
BARK2	inactive (98.95%)	-
GPRK4	inactive (84.5%)	-
GPRK5	inactive (71.61%)	inactive (61%)
GPRK6	inactive (72.03%)	inactive (87%)
GPRK7	inactive (60.41%)	inactive (58%)

AGC - RSK

Bioactivity
Kinase	RBC	Millipore
MSK1	active (16.99%)	active (24%)
MSK2	active (34.92%)	active (18%)
p70S6K	active (11.56%)	active (11%)
p70S6Kb	inactive (58.97%)	-
RSK2	active (2.47%)	active (1%)
RSK4	active (3.97%)	active (4%)
RSK1	active (4.67%)	active (1%)
RSK3	active (1.4%)	active (15%)

AGC - PKA

Bioactivity
Kinase	RBC	Millipore
PKACg	-	-
PRKX	inactive (85.3%)	inactive (103%)
PKACa	inactive (71.97%)	inactive (60%)

AGC - PKC

Bioactivity
Kinase	RBC	Millipore
PKCa	active (6.27%)	active (1%)
PKCd	active (4.73%)	active (2%)
PKCe	active (0.49%)	active (10%)
PKCh	active (7.67%)	active (9%)
PKCg	active (17.6%)	active (1%)
PKCi	inactive (55.95%)	inactive (74%)
PKCt	active (17.28%)	active (3%)
PKCz	inactive (57.24%)	inactive (72%)
PKCb	active (12.59%)	-

AGC - PKG

Bioactivity
Kinase	RBC	Millipore
PKG2	active (38.67%)	-
PKG1	active (38.33%)	-

AGC - PKN

Bioactivity
Kinase	RBC	Millipore
PKN1	-	-
PKN2	inactive (72.1%)	active (23%)

AGC - SGK

Bioactivity
Kinase	RBC	Millipore
SGK1	active (48.56%)	inactive (60%)
SGK2	inactive (74.41%)	active (41%)
SGK3	inactive (72.76%)	inactive (101%)

AGC - NDR

Bioactivity
Kinase	RBC	Millipore
NDR1	active (0%)	-

TK

Test Member No. in Family
Family	no. Member on 518kinase	RBC	Millipore
Abl	2	2	2
Ack	2	1	1
ALK	2	2	1
Axl	3	3	3
Src	11	11	10
Tec	5	5	4
PDGFR	5	5	5
Csk	2	2	1
DDR	2	1	1
EGFR	4	3	2
Eph	14	12	11
FAK	2	2	2
Fer	2	2	2
FGFR	4	4	4
VEGFR	3	3	3
InsR	3	3	3
JakA	4	4	2
Musk	1	1	1
Ret	1	1	1
Met	2	2	2
Sev	1	1	1
Syk	2	2	2
Tie	2	1	1
Trk	3	3	2

TK - Abl

Bioactivity
Kinase	RBC	Millipore
ABL1	inactive (102.69%)	inactive (108%)
ABL2	inactive (96.25%)	inactive (90%)

TK - Ack

Bioactivity
Kinase	RBC	Millipore
ACK	inactive (94.68%)	inactive (112%)

TK - ALK

Bioactivity
Kinase	RBC	Millipore
ALK	inactive (86.96%)	inactive (94%)
LTK	inactive (99.48%)	-

TK - Axl

Bioactivity
Kinase	RBC	Millipore
AXL	inactive (100.14%)	inactive (70%)
MER	inactive (92.15%)	inactive (60%)
TYRO3	inactive (97.23%)	inactive (96%)

TK - Src

Bioactivity
Kinase	RBC	Millipore
BLK	inactive (86.96%)	inactive (65%)
BRK	inactive (95.27%)	inactive (110%)
SRC	inactive (92.59%)	inactive (82%)
FGR	inactive (90.9%)	inactive (96%)
FRK	inactive (92.32%)	inactive (102%)
FYN	inactive (88.26%)	inactive (102%)
HCK	inactive (92.32%)	inactive (74%)
LCK	inactive (91.83%)	inactive (109%)
SRM	inactive (103.78%)	-
YES	inactive (84.6%)	inactive (105%)
LYN	inactive (84.15%)	inactive (79%)

TK - Tec

Bioactivity
Kinase	RBC	Millipore
BMX	inactive (101.23%)	inactive (91%)
BTK	inactive (93.78%)	inactive (89%)
ITK	inactive (101.56%)	inactive (102%)
TEC	inactive (115.96%)	-
TXK	inactive (106.51%)	inactive (97%)

TK - PDGFR

Bioactivity
Kinase	RBC	Millipore
KIT	inactive (69.52%)	inactive (115%)
FLT3	active (5.6%)	active (10%)
FMS	inactive (76.74%)	active (50%)
PDGFRb	inactive (78.14%)	inactive (106%)
PDGFRa	inactive (69.2%)	inactive (121%)

TK - Csk

Bioactivity
Kinase	RBC	Millipore
CSK	inactive (96.95%)	inactive (122%)
CTK	inactive (100.37%)	-

TK - DDR

Bioactivity
Kinase	RBC	Millipore
DDR2	inactive (110.98%)	inactive (113%)

TK - EGFR

Bioactivity
Kinase	RBC	Millipore
EGFR	inactive (94.05%)	inactive (103%)
ErbB2	inactive (96.52%)	-
ErbB4	inactive (87.68%)	inactive (113%)

TK - Eph

Bioactivity
Kinase	RBC	Millipore
EphA1	inactive (89.94%)	inactive (95%)
EphA2	inactive (81.84%)	inactive (102%)
EphA3	inactive (93.01%)	inactive (109%)
EphA4	inactive (88.16%)	inactive (112%)
EphA5	inactive (91.96%)	inactive (123%)
EphA6	-	-
EphA7	inactive (97.01%)	inactive (108%)
EphA8	inactive (102.5%)	inactive (120%)
EphB1	inactive (87.44%)	inactive (96%)
EphB2	inactive (100.1%)	inactive (119%)
EphB3	inactive (95.25%)	inactive (108%)
EphB4	inactive (86.82%)	inactive (124%)

TK - FAK

Bioactivity
Kinase	RBC	Millipore
FAK	inactive (86.07%)	inactive (133%)
PYK2	inactive (98.52%)	inactive (115%)

TK - Fer

Bioactivity
Kinase	RBC	Millipore
FER	inactive (69.79%)	inactive (113%)
FES	inactive (90.86%)	inactive (125%)

TK - FGFR

Bioactivity
Kinase	RBC	Millipore
FGFR1	inactive (74.08%)	active (50%)
FGFR2	inactive (95.36%)	inactive (88%)
FGFR3	inactive (87.9%)	inactive (94%)
FGFR4	inactive (88.99%)	inactive (121%)

TK - VEGFR

Bioactivity
Kinase	RBC	Millipore
FLT1	inactive (78.08%)	active (14%)
FLT4	active (30.57%)	active (7%)
KDR	inactive (65.46%)	inactive (61%)

TK - InsR

Bioactivity
Kinase	RBC	Millipore
IGF1R	inactive (91.66%)	inactive (127%)
IRR	inactive (91.26%)	inactive (137%)
INSR	inactive (88.86%)	inactive (115%)

TK - JakA

Bioactivity
Kinase	RBC	Millipore
JAK1	inactive (88.67%)	-
JAK2	inactive (93.94%)	inactive (121%)
JAK3	inactive (81.46%)	inactive (92%)
TYK2	inactive (79.3%)	-

TK - Musk

Bioactivity
Kinase	RBC	Millipore
MUSK	inactive (71.94%)	inactive (134%)

TK - Ret

Bioactivity
Kinase	RBC	Millipore
RET	inactive (93.38%)	inactive (95%)

TK - Met

Bioactivity
Kinase	RBC	Millipore
RON	inactive (109.42%)	inactive (102%)
MET	inactive (101.91%)	inactive (98%)

TK - Sev

Bioactivity
Kinase	RBC	Millipore
ROS	inactive (62.75%)	inactive (108%)

TK - Syk

Bioactivity
Kinase	RBC	Millipore
SYK	inactive (89.97%)	inactive (120%)
ZAP70	inactive (93.01%)	inactive (133%)

TK - Tie

Bioactivity
Kinase	RBC	Millipore
TIE2	inactive (99.74%)	inactive (120%)

TK - Trk

Bioactivity
Kinase	RBC	Millipore
TRKA	inactive (107.57%)	active (48%)
TRKB	inactive (55.82%)	active (36%)
TRKC	active (20.24%)	-

TKL

Test Member No. in Family
Family	no. Member on 518kinase	RBC	Millipore
STKR	12	5	2
RAF	5	3	1
IRAK	4	2	2
LISK	4	1	1
LRRK	2	1	-
MLK	10	5	2
RIPK	5	1	1

TKL - STKR

Bioactivity
Kinase	RBC	Millipore
ALK1	inactive (106.55%)	-
ALK2	inactive (96.33%)	-
ALK4	inactive (105.92%)	inactive (119%)
TGFbR1	inactive (101.71%)	inactive (91%)
TGFbR2	inactive (90.39%)	-

TKL - RAF

Bioactivity
Kinase	RBC	Millipore
ARAF	inactive (100.25%)	-
BRAF	inactive (103.44%)	-
RAF1	inactive (118.32%)	inactive (113%)

TKL - IRAK

Bioactivity
Kinase	RBC	Millipore
IRAK1	inactive (91.11%)	inactive (111%)
IRAK4	inactive (135.1%)	inactive (121%)

TKL - LISK

Bioactivity
Kinase	RBC	Millipore
LIMK1	inactive (94.72%)	inactive (117%)

TKL - LRRK

Bioactivity
Kinase	RBC	Millipore
LRRK2	inactive (89.85%)	-

TKL - MLK

Bioactivity
Kinase	RBC	Millipore
MLK1	active (45.99%)	inactive (58%)
MLK2	inactive (81.77%)	-
MLK3	inactive (61.03%)	-
TAK1	inactive (93.64%)	inactive (103%)
ZAK	inactive (93.06%)	-

TKL - RIPK

Bioactivity
Kinase	RBC	Millipore
RIPK2	inactive (87.72%)	inactive (90%)

CAMK

Test Member No. in Family
Family	no. Member on 518kinase	RBC	Millipore
CAMKL	20	13	12
CAMK1	5	5	3
CAMK2	4	4	3
RAD53	1	1	1
DAPK	5	4	4
DCAMKL	3	1	1
MAPKAPK	5	5	4
MLCK	5	2	1
PHK	2	2	1
PIM	3	3	3
PKD	3	3	2
TSSK	5	2	2
STK33	1	-	-

CAMK - CAMKL

Bioactivity
Kinase	RBC	Millipore
NuaK1	active (25.92%)	active (35%)
BRSK1	inactive (76.91%)	active (42%)
BRSK2	inactive (55.61%)	inactive (71%)
CHK1	active (39.09%)	inactive (54%)
LKB1	inactive (92.82%)	inactive (105%)
MARK1	inactive (84.13%)	inactive (65%)
MARK3	inactive (71.96%)	-
MARK4	inactive (66.87%)	-
MELK	active (41.99%)	active (41%)
PASK	inactive (67.01%)	inactive (74%)
QIK	inactive (94.71%)	-
NuaK2	inactive (65.98%)	-
MARK2	inactive (71.71%)	inactive (67%)
AMPKa1	-	active (44%)
AMPKa2	-	active (26%)
SIK	-	inactive (71%)

CAMK - CAMK1

Bioactivity
Kinase	RBC	Millipore
CaMK1a	inactive (82.68%)	inactive (89%)
CaMK1b	inactive (106.48%)	-
CaMK1d	inactive (67.96%)	inactive (63%)
CaMK1g	inactive (97.35%)	-
CaMK4	inactive (89.54%)	inactive (88%)

CAMK - CAMK2

Bioactivity
Kinase	RBC	Millipore
CaMK2a	active (35.21%)	-
CaMK2b	inactive (74.47%)	inactive (63%)
CaMK2d	active (38.68%)	active (16%)
CaMK2g	inactive (73.26%)	active (28%)

CAMK - RAD53

Bioactivity
Kinase	RBC	Millipore
CHK2	inactive (68.48%)	inactive (52%)

CAMK - DAPK

Bioactivity
Kinase	RBC	Millipore
DAPK1	inactive (87.91%)	inactive (106%)
DAPK2	inactive (105.09%)	inactive (72%)
DRAK1	inactive (86.93%)	inactive (108%)
DAPK3	inactive (84.54%)	inactive (62%)

CAMK - DCAMKL

Bioactivity
Kinase	RBC	Millipore
DCLK2	inactive (95.03%)	inactive (96%)

CAMK - MAPKAPK

Bioactivity
Kinase	RBC	Millipore
MAPKAPK2	inactive (85.43%)	inactive (108%)
MAPKAPK3	inactive (95.34%)	inactive (137%)
MAPKAPK5	inactive (93.74%)	inactive (125%)
MNK1	inactive (81.22%)	-
MNK2	inactive (85.35%)	inactive (93%)

CAMK - MLCK

Bioactivity
Kinase	RBC	Millipore
smMLCK	active (23.23%)	active (20%)
skMLCK	inactive (73.88%)	-

CAMK - PHK

Bioactivity
Kinase	RBC	Millipore
PHKg1	active (31.93%)	-
PHKg2	inactive (97.87%)	inactive (88%)

CAMK - PIM

Bioactivity
Kinase	RBC	Millipore
PIM1	active (5.3%)	active (1%)
PIM2	inactive (64.09%)	active (15%)
PIM3	active (1.9%)	active (22%)

CAMK - PKD

Bioactivity
Kinase	RBC	Millipore
PKD1	inactive (71.63%)	inactive (108%)
PKD3	inactive (54.6%)	-
PKD2	inactive (78.84%)	inactive (97%)

CAMK - TSSK

Bioactivity
Kinase	RBC	Millipore
TSSK1	inactive (89.85%)	inactive (86%)
TSSK2	inactive (97.27%)	inactive (103%)

CAMK - STK33

Bioactivity
Kinase	RBC	Millipore
STK33	inactive (62.9%)	inactive (79%)

STE

Test Member No. in Family
Family	no. Member on 518kinase	RBC	Millipore
STE11	8	3	1
STE-Unique	2	2	-
STE20	31	23	14
STE7	7	3	3

STE - STE11

Bioactivity
Kinase	RBC	Millipore
MAP3K5	inactive (90.24%)	inactive (96%)
MAP3K2	inactive (82.78%)	-
MAP3K3	inactive (96.98%)	-

STE - STE-Unique

Bioactivity
Kinase	RBC	Millipore
COT	inactive (93.93%)	-
NIK	inactive (110.46%)	-

STE - STE20

Bioactivity
Kinase	RBC	Millipore
GCK	inactive (94.17%)	inactive (75%)
HGK	inactive (55.93%)	-
KHS1	inactive (68.48%)	-
LOK	active (19.38%)	active (33%)
MINK	inactive (92.1%)	inactive (84%)
MST1	active (25.05%)	active (40%)
MST2	active (44.23%)	inactive (73%)
MST3	inactive (52.9%)	active (27%)
MST4	active (37.2%)	-
OSR1	inactive (97.38%)	-
PAK1	inactive (84.16%)	-
PAK2	inactive (96.18%)	inactive (115%)
PAK3	inactive (92.07%)	inactive (90%)
PAK4	inactive (93.62%)	inactive (102%)
PAK5	inactive (92.76%)	inactive (88%)
PAK6	inactive (100.5%)	inactive (95%)
SLK	active (45.83%)	-
YSK1	inactive (69.72%)	-
STLK3	inactive (91.89%)	-
TAO1	inactive (80.77%)	inactive (97%)
TAO2	inactive (75.72%)	inactive (101%)
TAO3	inactive (67.65%)	inactive (88%)
MYO3B	active (0%)	-

STE - STE7

Bioactivity
Kinase	RBC	Millipore
MAP2K1	inactive (83.63%)	inactive (109%)
MAP2K2	inactive (98.86%)	-
MAP2K6	inactive (97.39%)	inactive (93%)
MAP2K7	-	inactive (79%)

Other

Test Member No. in Family
Family	no. Member on 518kinase	RBC	Millipore
Aur	3	3	3
CAMKK	2	2	-
Haspin	1	1	1
IKK	4	4	3
NEK	11	8	5
TOPK	1	1	-
PLK	4	3	3
SgK496	1	1	-
NAK	4	1	-
TTK	1	1	-
ULK	5	3	2
WEE	3	1	-
WNK	4	2	2
TLK	2	1	1

Other - Aur

Bioactivity
Kinase	RBC	Millipore
AurA	inactive (107.54%)	inactive (100%)
AurB	inactive (86.6%)	active (31%)
AurC	inactive (84.74%)	inactive (109%)

Other - CAMKK

Bioactivity
Kinase	RBC	Millipore
CaMKK1	inactive (90.79%)	-
CaMKK2	inactive (59.78%)	-

Other - Haspin

Bioactivity
Kinase	RBC	Millipore
Haspin	inactive (93.57%)	active (43%)

Other - IKK

Bioactivity
Kinase	RBC	Millipore
IKKa	inactive (104.85%)	inactive (101%)
IKKb	inactive (118.7%)	inactive (104%)
IKKe	inactive (89.43%)	-
TBK1	inactive (83.83%)	inactive (80%)

Other - NEK

Bioactivity
Kinase	RBC	Millipore
NEK1	inactive (92.51%)	-
NEK11	inactive (104.14%)	inactive (72%)
NEK2	inactive (96.24%)	inactive (94%)
NEK4	inactive (94.27%)	-
NEK6	inactive (96.32%)	inactive (96%)
NEK7	inactive (87.14%)	inactive (117%)
NEK9	inactive (99.99%)	-
NEK3	inactive (109.87%)	inactive (98%)

Other - TOPK

Bioactivity
Kinase	RBC	Millipore
PBK	inactive (81.28%)	-

Other - PLK

Bioactivity
Kinase	RBC	Millipore
PLK1	inactive (100.76%)	inactive (114%)
PLK2	inactive (97.3%)	inactive (113%)
PLK3	inactive (94.94%)	inactive (101%)

Other - SgK496

Bioactivity
Kinase	RBC	Millipore
SgK496	inactive (94.99%)	-

Other - NAK

Bioactivity
Kinase	RBC	Millipore
MPSK1	inactive (80.81%)	-

Other - TTK

Bioactivity
Kinase	RBC	Millipore
TTK	inactive (98.24%)	-

Other - ULK

Bioactivity
Kinase	RBC	Millipore
ULK1	inactive (84.73%)	-
ULK2	inactive (145.09%)	inactive (110%)
ULK3	active (0%)	inactive (101%)

Other - WEE

Bioactivity
Kinase	RBC	Millipore
Wee1	inactive (101.34%)	-

Other - WNK

Bioactivity
Kinase	RBC	Millipore
Wnk2	inactive (107.41%)	inactive (120%)
Wnk3	inactive (98.38%)	inactive (121%)

Other - TLK

Bioactivity
Kinase	RBC	Millipore
TLK2	active (0%)	active (21%)

CK1

Test Member No. in Family
Family	no. Member on 518kinase	RBC	Millipore
CK1	7	6	4
VRK	3	1	1

CK1 - CK1

Bioactivity
Kinase	RBC	Millipore
CK1d	inactive (97.56%)	inactive (90%)
CK1g1	inactive (99.12%)	inactive (107%)
CK1g2	inactive (90.33%)	inactive (101%)
CK1g3	inactive (96.87%)	inactive (83%)
CK1a	inactive (96.46%)	-
CK1e	inactive (96.3%)	-

CK1 - VRK

Bioactivity
Kinase	RBC	Millipore
VRK1	inactive (95.48%)	-
VRK2	-	inactive (119%)

CMGC

Test Member No. in Family
Family	no. Member on 518kinase	RBC	Millipore
CK2	3	2	2
CLK	4	4	2
DYRK	10	9	4
MAPK	14	10	10
GSK	2	2	2
SRPK	3	3	3

CMGC - CK2

Bioactivity
Kinase	RBC	Millipore
CK2a1	inactive (99.53%)	inactive (107%)
CK2a2	inactive (92.01%)	inactive (95%)

CMGC - CLK

Bioactivity
Kinase	RBC	Millipore
CLK1	inactive (60.29%)	-
CLK2	active (28.02%)	active (14%)
CLK3	inactive (94.33%)	inactive (66%)
CLK4	active (44.83%)	-

CMGC - DYRK

Bioactivity
Kinase	RBC	Millipore
DYRK1A	inactive (54.69%)	-
DYRK1B	inactive (76.02%)	-
DYRK2	inactive (111.03%)	inactive (103%)
DYRK3	inactive (97.91%)	-
DYRK4	inactive (95.03%)	-
HIPK1	inactive (96.55%)	inactive (90%)
HIPK2	inactive (77.54%)	inactive (69%)
HIPK3	inactive (102.12%)	inactive (87%)
HIPK4	inactive (112.72%)	-

CMGC - MAPK

Bioactivity
Kinase	RBC	Millipore
Erk1	inactive (107.58%)	inactive (101%)
Erk2	inactive (100.12%)	inactive (98%)
JNK3	inactive (99.3%)	inactive (117%)
NLK	inactive (93.97%)	inactive (127%)
p38a	inactive (90.72%)	inactive (109%)
p38b	inactive (96.06%)	inactive (106%)
p38d	inactive (96.83%)	inactive (126%)
p38g	inactive (95.87%)	inactive (125%)
JNK1	inactive (90.22%)	inactive (111%)
JNK2	inactive (98.71%)	inactive (100%)

CMGC - GSK

Bioactivity
Kinase	RBC	Millipore
GSK3A	active (11.17%)	active (18%)
GSK3B	active (7.01%)	active (24%)

CMGC - SRPK

Bioactivity
Kinase	RBC	Millipore
MSSK1	inactive (86.88%)	inactive (113%)
SRPK1	inactive (87.02%)	inactive (130%)
SRPK2	inactive (94.36%)	inactive (98%)

Atypical

Test Member No. in Family
Family	no. Member on 518kinase	RBC	Millipore
PDHK	5	1	-
Alpha	6	-	1
PIKK	6	-	1

Atypical - PDHK

Bioactivity
Kinase	RBC	Millipore
PDHK1	inactive (83.31%)	-

Atypical - Alpha

Bioactivity
Kinase	RBC	Millipore
eEF2K	-	inactive (113%)

Atypical - PIKK

Bioactivity
Kinase	RBC	Millipore
FRAP	-	inactive (84%)